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CoMBI-S(Sliding microtome, manual/滑走式ミクロトーム、手動)

Paraffin block

See "Paraffin block"

Frozen block

See "Frozen specimen"

Camera settings/カメラの設定

See "Camera settings" for detailed information about exposure time, iris, and others.

露出や絞りなど、カメラの設定については、Camera Settingsをご覧ください。

Operation

CoMBI-S manual type

See Sci Rep (2021)11:13108, Suppl. Fig. S4 for detail.

  1. Connect camera and sensor to the controller box.
  2. Place the sensor unit under the handle.
  3. Expose blockface as a start position.
  4. Turn ON camera controll (Red button).
  5. Turn ON HOLD button. This means half-push of the shutter button on the camera (fix focus).
  6. Capture a scale by pushing "RELEASE" button.
  7. Slice and image blockface serially.

NOTE: Shutter is released when the handle is going back. Thus, move the handle slowly and carefully when going back. At the magnification 1 - 3x, you do not need care about stability. However, at the highest magnification (5x), blur-free imaging is possible, but very careful manual operation is required.